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CLARIFY. Clarification technique and multiphoton microscopy

CLARIFY. Clarification technique and multiphoton microscopy

CLARIFY, tissue clarification technique in combination with multiphoton microscopy, consists of a chemical treatment of the tissues for the polymerization of their proteins and the elimination of all their lipids in order to turn them into a completely transparent tissue. With this clarification we improve the penetration capacity of the light into the tissues and therefore its optical quality improving the quality of 3D images taken by multiphoton excitation fluorescence optical microscopy (MP).

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